The primary aim of this study was to investigate whether acacia gum (GUM) qualifies as a prebiotic fiber and to assess how well it is tolerated by the digestive system of healthy individuals. The study compared the effects of increasing doses of GUM to those of sucrose (SUC) in terms of stool production, the concentration of major bacterial groups in the stools, and the occurrence and intensity of digestive symptoms (such as flatulence, bloating, abdominal cramps, and diarrhea). Over a 10-day period, the consumption of GUM at doses of 10 and 15 grams per day led to an increase in the counts of lactic acid-producing bacteria and bifidobacteria in the stools, while it had no significant impact on the total counts of anaerobic and aerobic bacteria. This selective effect was more pronounced in individuals with initially low levels of bifidobacteria in their feces. The study found that GUM was highly digestible, with about 95% being broken down in the digestive process. Its estimated caloric value ranged from 5.5 to 7.7 kJ/g. Additionally, GUM consumption increased stool weight by 30% due to higher fecal water content. The digestive tolerance of GUM was found to be excellent and comparable to that of SUC up to a daily dose of 30 g. Beyond this dose, the primary complaint was an increase in flatulence. However, even at doses exceeding 50 g/day, the severity of symptoms remained mild, with other intestinal issues being infrequently reported. In summary, GUM is a well-tolerated dietary fiber with properties that support the growth of bifidobacteria and are believed to contribute positively to intestinal health.